نوع مقاله : مقاله پژوهشی
نویسندگان
1 عضو هیات علمی گروه باغبانی، دانشکده کشاورزی، دانشگاه شاهد تهران
2 دانشجوی سابق کارشناسی ارشد گروه باغبانی، دانشکده کشاورزی، دانشگاه شاهد تهران
3 دانشجوی سابق کارشناسی ارشد گروه باغبانی، دانشکده کشاورزی، دانشگاه شاهد تهران.
4 عضو هیات علمی گروه باغبانی، دانشکده کشاورزی، دانشگاه شاهد تهران.
کلیدواژهها
عنوان مقاله English
نویسندگان English
Conducted in 2023 at Shahed University's Horticulture Laboratory, Tehran, Iran, the experiment used mature D. lotus seeds from Taleghan, sterilized with 1% sodium hypochlorite for 10 minutes, and rinsed. Treatments: 0 mM (control), 25 mM, 50 mM, 100 mM, 200 mM NaCl. Seeds (20/replicate) in 15-cm Petri dishes with Whatman No. 1 filter paper, moistened with 10 mL solution, incubated at 25 ± 2°C, 70 ± 5% RH, 16/8-hour light/dark for 21 days. Solutions refreshed bi-daily. Completely randomized design, three replicates.
Parameters: Germination percentage (14 days, radicle emergence); growth traits (leaf, stem, root lengths on 10 seedlings via digital caliper); seedling fresh/dry weights (dried 70°C, 48 hours); electrolyte leakage (conductivity pre/post 95°C heat, (C1/C2) × 100); chlorophyll content (SPAD-502 on three leaves); chlorophyll fluorescence (F0, Fm, Fv/Fm via PAM-2500 after 20-min dark adaptation).
Data normalized, ANOVA via SAS 9.3; means compared by Duncan's test (P ≤ 0.05); graphs in Excel 2021.
کلیدواژهها English
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