Keluss is a member of the umbelliferous family and a car plant with medicinal properties, which is on the verge of extinction in recent years due to improper exploitation and uprooting and bud picking before seeding. This species grows in cold and mountainous bioclimates and is used in traditional medicine for the treatment of various diseases such as cardiovascular diseases, treatment of gastric and respiratory ulcers, and intestinal inflammation. For this purpose, an experiment was conducted to investigate the characteristics of the biological agent and the active ingredients of Keluss medicinal plant in the greenhouse of Shahid University Medicinal Plants Research Center in 2022. Greenhouse experiment included fertilizer treatments (no fertilizer application, chemical fertilizer with nitrogen source and nitrous biofertilizer) in three replicates in the form of pot culture with cocopeat + percolite + peat moss. According to the results of the present research, growth indicators such as dry weight of shoot, plant height, soluble compounds, secondary metabolites and DPPH level were affected by fertilizer treatment. Nitrozist biofertilizer increases physiological traits such as total phenol (2.55 mg/g FW), flavonoid content in three wavelengths and carbohydrate content (4.4 mg/g FW) and antioxidant capacity (28.63%) became Keluss. The application of nitrogenous biofertilizer and the combination of chemical fertilizer treatment of 80 kg per hectare combined with biofertilizer also had a positive effect on root characteristics, flavonoid content and antioxidant capacity of leaves of Keluss medicinal plant.