Document Type : Original Article
Authors
1 Eqbal Lahori Institute
2 Shahed University
3 Islamic Azad University, Shirvan branch
4 Gorgan University of Agricultural Sciences and Natural Resources
Abstract
Aloe vera gel and extract has many biological and physiological properties and is used in various pharmaceutical, food, cosmetic and health industries. In this study, in order to determine the amounts of total tannin, total phenol, total flavonoid and antioxidant of Aloe vera L. hydroalcoholic extract, an experiment was conducted in a completely randomized design with three replications in 2019.
This research was conducted in a greenhouse located in Dehshak village of Mashhad city, Razavi Khorasan province. A methanolic extract was prepared from the gel of yellow patience leaves. The amount of phenol and flavonoid was measured by spectrometry and the antioxidant activity of the gel extract was measured using DPHH free radical. According to the results obtained from determining the amounts of secondary metabolites and antioxidant activity, the amount of total phenol in the hydroalcoholic extract of yellow patience gel is 90.04 microequivalent grams of gallic acid per milligram dry weight of the gel, the total flavonoid content is 66.25 microequivalent grams. Quercetin per mg dry weight of the gel and total tannin content was 87.43 microequivalent grams of catechin per mg dry weight of the gel. The measurement of antioxidant activity in three methods, DPPH, FRAP and BCB, had a significant positive correlation with total phenol, total flavonoid and total tannin content. The results of this study showed that yellow patience gel has high antioxidant activity and also has a direct relationship with the amount of phenolic and flavonoid compounds.
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